The video demonstrates how to count red blood cells (RBCs) in a blood sample using a hemocytometer by preparing the RBC pipette, diluting the blood sample, loading the counting chamber, observing and counting the cells under a microscope, and calculating the total RBC count per cubic millimeter. Normal RBC count ranges for men and women, as well as conditions leading to low or high RBC counts, are mentioned. Platelet cell counting is briefly covered with a different diluting solution, discussing normal platelet count range and potential causes of low or high platelet counts.
### Methodology
1. Prepare the RBC pipette and dilute the blood sample.
2. Load the counting chamber with the diluted blood sample.
3. Allow the RBCs to settle and observe them under a microscope.
4. Count the cells in specific squares of the counting grid.
5. Calculate the total RBC count per cubic millimeter using the provided formula.
6. Repeat a similar process for counting platelet cells using a different diluting solution.
### Speakers
Not specified in the subtitles.
Notable Quotes
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00:05
— « In this video we're going to count the number of red blood cells in a sample of human blood using the hemocytometer.
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00:14
— « for this experiment you will need the following: counting chamber, RBC pipette, blood sample, RBC diluting solution, compound-like microscope, cotton, and rubbing alcohol.
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02:16
— « once this is done prepare the counting chamber for sample loading.
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03:59
— « locate the 1st 5th 13th 21st and 25th smaller squares in the RBC grid.
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05:58
— « normal RBC count for adult men and women is as follows: in men it's 4.7 to 6.1 million cells per cubic millimeter, in women it's 4.2 to 5.4 million cells per cubic millimeter. »