Summary of Bacterial Transformation Principle Using pGLO plasmid Tagalog Filipino Molecular Biology BMLS
Bacterial transformation using the pGLO plasmid can transform E. coli into a glowing organism under ultraviolet light.
Genetically modified organisms have various applications, including fighting diseases, fortifying nutrients, and producing insulin, hormones, vaccines, and anti-cancer drugs.
The process involves inserting a gene into a plasmid, putting it into bacteria, allowing the bacteria to multiply, and producing the desired protein.
The pGLO plasmid contains genes for antibiotic resistance and green fluorescent protein (GFP).
The transformation process involves shielding the plasmid's negative charge with calcium chloride, heat shocking the bacteria to open pores, incubating with LB broth for gene expression, and selecting for transformed bacteria on ampicillin plates.
The presence of arabinose activates the GFP gene, leading to the production of green fluorescent protein.
The efficiency of transformation can be calculated by dividing the total number of colonies by the amount of DNA spread on the agar plates.
Speakers
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Notable Quotes
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— 35:34 — « ", "to allow the pores to be open and and immediately"], [" »
— 35:59 — « ", "protein so allows the expression of your beta"], [" »
— 38:05 — « ", "with arabinose the switch is on rsc"], [" »
— 39:22 — « ", "green fluorescent light"] »
Category
Educational