Summary of Thin Layer Chromatography (TLC)-Animation- Chromatographie sur Couche Mince (CCM)

Scientific Concepts

Thin Layer Chromatography (TLC): A method for separating and identifying compounds in a mixture.
Mobile Phase: A solvent or mixture of solvents that moves up the TLC plate.
Stationary Phase: A plate coated with an adsorbent material (e.g., silica gel, aluminum oxide, or cellulose).
Capillary Action: The process by which the solvent moves up the plate.
Differential Partitioning: Separation of components based on their affinity for the mobile and stationary phases.
Retention Factor (RF): A value calculated by dividing the distance traveled by a compound by the distance traveled by the solvent front.

Draw a pencil line 1.5 cm from the bottom edge of the TLC plate.
Label the plate to mark the original position of the sample drop.
Apply a small drop of the sample mixture and known compounds on the baseline.
Place the TLC plate in a chamber with solvent, ensuring spots do not touch the eluent.
Allow the solvent to rise by Capillary Action until it nearly reaches the top of the plate.
Remove the plate before the solvent front reaches the top and mark the solvent front.
Dry the plate in ambient air or with warm air.
Visualize the spots under UV light or with chemical reagents if the compounds are colorless.
Mark the positions of the spots and calculate the RF values.
Compare RF values of unknown compounds with known standards to identify components.

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